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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has long been noticed in lots of tumors, which results in chromosome instability, and so could contribute to tumorigenesis. This protein was identified to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a crucial position in advertising and marketing cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are actually explained. Two more splice variants were reported but comprehensive nucleotide sequence facts will not be still readily available.

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Blocking peptides are peptides that bind especially towards the goal antibody and block antibody binding. These peptide generally includes the epitope acknowledged through the antibody.

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This manual outlines further more doable optimization of mobile and immunoassay parameters to be certain the absolute best results are...

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This guideline outlines even further doable optimization of cellular and immunoassay parameters to make sure the very best results are...

Antibodies bound to the blocking peptide no longer bind towards the epitope around the concentrate on protein. By comparing the staining in the blocked antibody as opposed to the antibody by yourself, one can see which staining is specific.

Focus on Information and facts Cyclin E belongs to the extremely conserved cyclin relatives, whose customers are characterised by a remarkable periodicity in protein abundance from the cell cycle. Cyclins purpose as regulators of CDK kinases. Distinctive cyclins show distinctive expression and degradation designs which lead to the temporal coordination of each and every mitotic celebration. Cyclin E types a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates within the G1-S section boundary which is degraded as cells development as a result of S section.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation here by equally antibodies...

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